The Bloomsbury Colleges | PhD Studentships | Studentships 2016 | Modelling the entry process in Human Cytomegalovirus using genomics from natural populations (Bbk / LSHTM)
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Modelling the entry process in Human Cytomegalovirus using Genomics from natural populations

Principle Supervisor: Dr Maya Topf (Bbk)

Co-Supervisor: Dr Ursula Gompels (LSHTM)

Human cytomegalovirus (HCMV),  is a common global pathogen, the dominant viral cause of congenital birth defects in the developed world and a rising vaccine priority. HCMV is also a major viral opportunistic infection in HIV/AIDS increasing morbidity and mortality. This is one of the largest DNA viruses, presenting challenges in understanding the remarkable complexity in its entry machinery as compared to smaller enveloped viruses (e.g. HIV or Influenza). A structural view of the entry process, will be a critical step in identifying potential novel therapeutics, including vaccines and drugs. The aim of this PhD is to build a structural model of the core HCMV fusion machinery during cellular infection by developing a mathematical/computational modelling approach that will combine data from natural transmission pathways, cryoET (in collaboration with the Grünewald lab , Oxford), X-ray crystallography, genomics and genotypic variation. Using this integrated approach the project aims to identify molecular mechanisms underlying HCMV entry stage into the host cell and therapeutic targets.

Candidate Requirements:

This PhD is aimed at graduates with a strong interest in multi-disciplinary research and especially in computational work. We invite applications from highly motivated students from a wide range of academic backgrounds including biological, biomedical, biochemical, cell biology, genomic, physical, computational, engineering or mathematical disciplines.

Key References:

[1] Gompels UA, Larke N, Sanz-Ramos M, Bates M, Musonda K, Manno D, Siame J, Monze, M, Filteau S; CIGNIS Study Group. (2012) Human cytomegalovirus infant infection adversely affects growth and development in maternally HIV-exposed and unexposed infants in Zambia. Clin Infect Dis. 54:434-42.

[2] Maurer UE, Zeev-Ben-Mordehai T, Pandurangan AP, Cairns TM, Hannah BP, Whitbeck JC, Eisenberg RJ, Cohen GH, Topf M, Huiskonen JT, Grünewald K. (2013). The structure of herpesvirus fusion glycoprotein B-bilayer complex reveals the protein-membrane and lateral protein-protein interaction. Structure 21:1-10.

[3] Bates M, Monze M, Bima H, Kapambwe M, Kasolo FC, Gompels UA, CIGNIS group. (2008) High human cytomegalovirus loads and diverse linked variable genotypes in both HIV-1 infected and exposed, but uninfected, children in Africa, Virology 382:28-36.

[4] Pandurangan AP, Vasishtan D, Alber F, Topf M (2015) γ-TEMPy: simultaneous fitting of components in 3D-EM maps of their assembly using a genetic algorithm. Structure 23:2365-76

Further details about the project may be obtained from:

Principle Supervisor: Dr Maya Topf,  (m.topf@mail.cryst.bbk.ac.uk)

http://www.bbk.ac.uk/biology/our-staff/academic/maya-topf

Co-Supervisor: Dr Ursula Gompels, (ursula.gompels@lshtm.ac.uk)

http://www.lshtm.ac.uk/aboutus/people/gompels.ursula

Further information about PhD's at Birkbeck is available from:

http://www.bbk.ac.uk/study/2016/phd/programmes/RMPBIOLG/

This studentship will be attached to the BBSRC LIDo programme. The successful student will be part of the Autumn 2016/17 cohort of students on the LIDo programme and will follow the LIDo programme structure in year 1. Further information about the LIDo programme are available below.

http://lido-dtp.ac.uk/

Application forms and details about how to apply are available from:

http://www.bbk.ac.uk/biology/study-here/studentships/Bloomsbury_Colleges_PhD_studentship_Human_Cytomegalovirus

Closing date for application is

Friday 26th February 2016, at 5pm